- The Kalinga cricket frog, first spotted from the Eastern Ghats in 2018, has now been recorded in the Western Ghats as well. This extends the distributional range of the species.
- The team compared the morphological features of this slightly larger frog found in Karnataka with those of other similar-looking Fejervarya/ Minervarya frogs. They also obtained a small portion of a single gene from two of the frogs for genetic analyses.
- These results reveal that the frog is indeed the Kalinga cricket frog. However, claims that this discovery signifies phenotypic plasticity in the species, or their having crossed biogeographic barriers, may not be possible to make with the data in hand.
Over the last decade alone, more than 100 frog species new to science have been discovered in India. One of them is the Kalinga cricket frog (Minervarya kalinga), a plain-looking brown amphibian that scientists discovered two years ago. What was special was that they had spotted it in the northern tracts of the Eastern Ghats, a hill range that has not seen as many frog discoveries as its western counterpart has.
Now the frog has popped up in the Western Ghats too, in Karnataka’s Uttara Kannada district. That’s more than 1,000 kilometres away from its eastern range. This proves that the Kalinga cricket frog may indeed be distributed across a larger area than what earlier, possibly incomplete surveys show. Since these frogs are also slightly larger than their eastern relatives, this could signify a new and morphologically distinct ‘population’ of frogs, postulate the scientists who made the discovery.
The cricket frogs of Asia
Size, colour and shape: looks, in general, are one of the many means to identify a frog. Especially so in the case of Asia’s cricket frogs. Cricket frogs (genus Fejervarya/ Minervarya) are found across large parts of Asia, including India. They were initially confused for pond frogs and placed in the genus Rana. A genus is a unit of classification containing organisms that share not only some common traits but also a common ancestor; it can contain several species.
In 1915, a naturalist found that some of these frogs in east India, Bangladesh and adjoining Asian countries, had two distinct lines running down their pale tummies; these were given their own genus, Fejervarya. In 2001, scientists found that some small Fejervarya-like frogs in Kerala and Karnataka looked different: they sported stark white upper lips, and a small gland called the rictal gland near their mouths apart from the fejervaryan lines on their bellies. So this frog species (species sahyadris) was assigned a new genus, Minervarya. With confusions surrounding all these frogs’ morphologies, however, another team grouped all south Asian fejeraryans (including Indian species) into another new genus, Zakerana. But confusions still prevailed.
A team (including K.P. Dinesh, currently with the Zoological Survey of India) then used basic genetic data to re-classify and re-name the genera Minervarya and Zakerana as Fejervaryas in 2015. In 2018, another team used several genes of fejervaryan frogs across 12 Asian countries and re-classified all southeast Asian frogs under the genus Fejervarya (including three species in east India that occur here as part of their Indo-China range), and those in south Asia into the genus Minervarya (all other Indian fejervarya-like frogs now fall in this category).
Amidst this fantastical taxonomic melee, scientists have been discovering new species of Minervarya across India. In 2018, a team including Dinesh stumbled across one from the Eastern Ghats, a hill range that runs roughly parallel to India’s east coast. Genetic studies revealed it to be a new species, the Kalinga cricket frog (now, Minervarya kalinga). The frog “is known with certainty” from many parts of Odisha including the Mahendragiri Hills, as well as from some areas in Andhra Pradesh, the team found.
But was it found elsewhere?
Part of that answer has surfaced now. When researcher Amit Hegde (a doctoral student at the Karnatak University), Dinesh and Girish Kadadevaru (associate professor at Karnatak University) surveyed for amphibians in the soppina bettas and surrounding agro-forests of Karnataka’s Uttara Kannada district, they came across a “Fejervarya-like” frog. Soppina bettas are ‘foliage’ forests – forest patches assigned to areca nut farmers to extract foliage, which is used as manure for areca palms.
“It looked different from the Fejervarya/ Minervarya frogs of the Western Ghats,” recalled Hegde.
Deceptive appearances?
The mystery frog resembled the Kalinga cricket frog, but it was slightly larger. Detailed measurements revealed that these frogs were around 5 centimetres long, around a centimetre longer than the Kalinga cricket frogs. When the team compared the specific dimensions of the mystery frog with two other cricket frog species in Uttara Kannada, as well as the Kalinga frogs from the Eastern Ghats, all four clustered neatly as separate units based on morphology alone.
So was the mystery frog a new species? Hegde and his colleagues turned to genetics, and sequenced a portion of a single gene (16S rRNA) from two of these mystery frogs. Visualising this data as a phylogenetic tree, the team found that the mystery frogs grouped under the eastern Kalinga cricket frogs. So they concluded that both were the same species – the Kalinga cricket frog.
While this not only extends the distribution of the species to the Western Ghats of India, the Kalinga cricket frogs here are a “morphologically variable and distinct population” of the species, argue the authors in their study published in the journal Zootaxa recently.
This morphological variation could signify “morphological phenotypic plasticity” – the phenomenon where an organism evolves differences in morphology in response to unique local environments, said Hegde.
The title of their research study (“Phenotypic divergence in large sized cricket frog species that crossed the geographical barriers within peninsular India”) also hints at a biogeographical inference, though this is not clarified further in the study.
However, the paper provides only a range extension of the species, commented amphibian biologist K.S. Seshadri, who was not involved in the study. The study does not provide evidence of population-level effects as no microsatellite data are used and no haplotypes are evident from the phylogenetic analysis, he wrote in an email to Mongabay-India. Moreover, it would be premature to allude the observed morphological variation to plasticity based on the study’s small sample size (two individuals for the genetic data and a simple ordination plot such as Principal Component Analysis of morphology using six samples), he added.
Findings like this range extension also “place the impetus on rigorous science”, wrote Seshadri. “These days, there is incredible evidence emphasizing the need for an integrative taxonomic approach that uses multiple, independent lines of evidence such as morphology, genetic data and acoustics.”
Providing frog call comparisons and examining multiple genes in the current study could have made a huge difference, he suggested.
Cricket frogs appear to be lodged in a much-contested taxonomic group. What do the confusing Minervarya-Fejervarya debates mean for these little frogs and their conservation?
“It can have huge implications, especially in the present world where science is not valued as much as it should be,” commented Seshadri. “Policymakers or managers could find this confusion infuriating and this may hinder the process of obtaining permits. Or a frog enthusiast may simply lose heart because of this confusion and may not be inclined to delve deep into the intellectual aspects of taxonomy. From a conservation standpoint, we have lost half the battle if we do not know what species is found where. So, our job as scientists is to make our science free of needless confusion or provide justification to the stands we take.”
CITATION:
Hegde et al 2020. Phenotypic divergence in large sized cricket frog species that crossed the geographical barriers within peninsular India. Zootaxa 4838 (2): 210-220.
Garg and Biju 2017. Description of four new species of Burrowing Frogs in the Fejervarya rufescens complex (Dicroglossidae) with notes on morphological affinities of Fejervarya species in the Western Ghats. Zootaxa 4277 (4): 451–490.
Sanchez et al 2018. Phylogeny and classification of fejervaryan frogs (Anura: Dicroglossidae). Salamandra 54 (2): 109-116.
Dinesh et al 2015. Systematic status of Fejervarya (Amphibia, Anura, Dicroglossidae) from South and SE Asia with the description of a new species from the Western Ghats of Peninsular India. Zootaxa 3999 (1): 079–094.
Prudhvi Raj et al 2018. Two new species of cricket frogs of the genus Fejervarya Bolkay. 1915 (Anura: Dicroglossidae) from the Peninsular India. Rec. Zool. Surv. India 118(1): 1-21.
Dubois et al 2001. A new genus and species of Ranidae (Amphibia, Anura) from south-western India. Alytes. Paris 19: 53–79.
Banner image: The Kalinga cricket frog from the Western Ghats. Photo by Amit Hegde.